Synthesis, Characterization and Safety Assessment of Zinc, Manganese and Copper Nanoparticles for Incorporation in Broiler Chicken Feed

Background: Zinc, manganese and copper are important trace minerals in broiler diet. Traditionally, these elements when generally supplemented as inorganic salts, their bioavailability is poor resulted mineral antagonism. To overcome issues, such were Nanoparticles due to higher efficacy. Hence, the current study was aimed synthesize, characterize evaluate cytotoxic effect of nano forms zinc, copper. Methods: Nano synthesized through physical method using planetary ball mill. In this particle size, shape, zeta potential elemental concentration determined by size analyser (PSA), transmission eectron microscope (TEM) inductively coupled plasma mass spectrometry (ICP-MS). The toxicity analysed MTT assay against vero cell line. Result: results revealed that nanoparticles mill (less than 100 nm) pure nature. Further, found be safe feed supplements inhibition rate dose dependent assay.